Cell Observation Device, Electrostimulation Device, and Cell Observation Method

Content Provider	The Lens
Abstract	A cell observation device is cell observation device for observing a cell held by a microplate having a well holding a sample including the cell and includes a microplate holder for holding the microplate thereon, an electrical stimulation unit including an electrode pair including a first electrode and a second electrode, and a position controller for controlling a position of the electrical stimulation unit in a state in which the first electrode is disposed closer to the center of the well than the second electrode when the electrode pair is disposed in the well of the microplate. The tip of the first electrode extends more than the tip of the second electrode.
Related Links	https://www.lens.org/lens/patent/009-295-682-184-79X/frontpage
Language	English
Publisher Date	2019-06-12
Access Restriction	Open
Alternative Title	Zellbeobachtungsvorrichtung, Elektrostimulationsvorrichtung Und Zellbeobachtungsverfahren Dispositif D'observation De Cellules, Dispositif D'électrostimulation Et Procédé D'observation De Cellules
Content Type	Text
Resource Type	Patent
Date Applied	2014-11-12
Agent	Hoffmann Eitle
Applicant	Hamamatsu Photonics Kk
Application No.	18209767
Claim	An electrical stimulation device for being inserted into a sample case having a holding unit holding a sample including a cell and applying electrical stimulation to the cell, the electrical stimulation device comprising: an electrode pair including a first electrode and a second electrode; and a position adjusting unit for adjusting a relative position of a tip of the first electrode to a tip of the second electrode. The electrical stimulation device according to claim 1, wherein the second electrode has a tubular shape, and the first electrode is disposed in the second electrode. The electrical stimulation device according to claim 1, wherein the second electrode includes a plurality of electrode members, and the first electrode is disposed between the plurality of electrode members. The electrical stimulation device according to any one of claims 1 to 3, wherein the first electrode has a pillar shape. The electrical stimulation device according to any one of claims 1 to 4, wherein a width of the tip of the first electrode is smaller than a width of the tip of the second electrode. A cell observation device for observing a cell held by a sample case including a holding unit holding a sample including the cell, the cell observation device comprising: a mounting unit for holding the sample case thereon; the electrical stimulation device according to any one of claims 1 to 5; and a position control unit for controlling a position of the electrode pair such that the electrode pair is disposed in the holding unit of the sample case. A cell observation method for observing a cell held by a sample case having a holding unit holding a sample including the cell, using an electrode pair including a first electrode and a second electrode, the cell observation method comprising: a step of adjusting a relative position of a tip of the first electrode to a tip of the second electrode; and a step of applying electrical stimulation to the sample using the electrode pair. The cell observation method according to claim 7, wherein the second electrode has a tubular shape, and the first electrode is disposed in the second electrode. The cell observation method according to claim 7, wherein the second electrode includes a plurality of electrode members, and the first electrode is disposed between the plurality of electrode members. The cell observation method according to any one of claims 7 to 9, wherein the first electrode has a pillar shape. The cell observation method according to any one of claims 7 to 10, wherein a width of the tip of the first electrode is smaller than a width of the tip of the second electrode. The cell observation method to any one of claims 7 to 11 further comprising: a step of holding the sample case on a mounting unit; and a step of controlling a position of the electrode pair such that the electrode pair is disposed in the holding unit of the sample case.
CPC Classification	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY;APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS; FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS; i.e. BIOREACTORS OR FERMENTERS Investigating Or Analysing Materials By Determining Their Chemical Or Physical Properties MICROORGANISMS OR ENZYMES;COMPOSITIONS THEREOF;PROPAGATING; PRESERVING; OR MAINTAINING MICROORGANISMS;MUTATION OR GENETIC ENGINEERING;CULTURE MEDIA
Extended Family	106-226-162-742-687 110-279-234-058-504 076-717-861-209-283 119-044-011-906-298 186-781-478-426-36X 067-372-035-252-784 078-436-864-371-392 169-879-395-322-898 037-629-277-804-951 116-985-570-606-940 009-295-682-184-79X 102-650-309-667-480 004-688-986-568-210 151-417-110-638-558 012-787-906-134-097 029-386-531-337-786
Patent ID	3495468
Inventor/Author	Saito Natsumi Ito Taira Kataoka Takuji
IPC	C12M1/42 C12Q1/02 G01N21/64
Status	Active
Simple Family	106-226-162-742-687 110-279-234-058-504 076-717-861-209-283 119-044-011-906-298 186-781-478-426-36X 067-372-035-252-784 078-436-864-371-392 169-879-395-322-898 037-629-277-804-951 116-985-570-606-940 009-295-682-184-79X 102-650-309-667-480 004-688-986-568-210 151-417-110-638-558 012-787-906-134-097 029-386-531-337-786
CPC (with Group)	C12M41/36 G01N21/6452 G01N33/4836 G01N33/48728 G01N27/416 C12N13/00 C12M35/02 G01N33/48707
Issuing Authority	United States Patent and Trademark Office (USPTO)
Kind	Patent Application Publication