A Sensor Body for Binding And/or Enriching And/or Detecting An Analyte in A Sample

Content Provider	The Lens
Abstract	The present invention relates to a sensor body for binding and/or enriching and analyte. Furthermore, the present invention relates to a method of binding an analyte to a sensor body. Furthermore, the present invention also relates to a method of enriching and/or washing an analyte bound to a sensor body and to a method of detecting an analyte in a sample.
Related Links	https://www.lens.org/lens/patent/009-275-671-001-662/frontpage
Language	English
Publisher Date	2019-07-03
Access Restriction	Open
Alternative Title	Sensorkörper Zur Bindung Und/oder Anreicherung Und/oder Detektion Eines Analyten in Einer Probe Corps De Capteur Pour Lier Et/ou Enrichir Et/ou Détecter Un Analyte Dans Un Échantillon
Content Type	Text
Resource Type	Patent
Date Applied	2017-12-29
Agent	Engelhard, Markus
Applicant	Blink Ag
Application No.	17211155
Claim	A sensor body for binding and/or enriching an analyte, said sensor body comprising a porous polymeric scaffold and an interstitial pore space within said polymeric scaffold, wherein said porous polymeric scaffold is composed of a polymer responsive to the change of at least one external condition to which said sensor body is exposed, e.g. pH, temperature, salt conditions, presence or absence of chemicals, wherein one or more capture agents for an analyte is/are attached to said porous polymeric scaffold. The sensor body according to claim 1, wherein said polymer responsive to the change of at least one external condition to which said sensor body is exposed, is a thermoresponsive polymer which is either a thermoresponsive polymer having a lower critical solution temperature (LCST polymer), preferably selected from poly( N- isopropylacrylamide) (pNIPAM), poly[2-(dimethylamino)ethyl methacrylate] (pDMAEMA), hydroxypropylcellulose, poly(vinylcaprolactame) (P(VCL), and polyvinyl methyl ether, or said thermoresponsive polymer is a thermoresponsive polymer having an upper critical solution temperature (UCST), preferably selected from poly(N-acryloyl glycinamide) (PNAGA), poly(allylamine)-co-poly(allylurea) and its derivatives, poly(methacrylamide), poly(N-acryloylaspargineamide), poly(N-methacryloylglutamineamide), poly(acrylamide)-co-(acrylonitrile). poly(sulfobetaine)s, poly(phosphorylcholine)s. The sensor body according to any of the foregoing claims, wherein said responsive polymer, in particular said thermoresponsive polymer is crosslinked, preferably either by at least one crosslinking reagent bridging and interconnecting between different polymer chains within said polymer, or by the provision of a substrate, in particular a substantially planar substrate, adjacent to said sensor body, and by interfacial crosslinking of different polymer chains of said polymer to said substrate, in particular to a surface of said substrate. The sensor body according to any of the foregoing claims, wherein said sensor body has the capability of reversibly adopting an expanded and a contracted state, and wherein, preferably, the interstitial pore space has a volume which is at least 50%, preferably at least 60%, more preferably at least 70%, even more preferably at least 80% and even more preferably at least 90% of the total volume of said sensor body when said sensor body is in said expanded state. The sensor body according to any of the foregoing claims, wherein approximately 10% - 90% of the total volume of said interstitial pore space are not accessible to said analyte, but are accessible to a solvent, preferably water. The sensor body according to any of the foregoing claims, wherein said analyte is a biomolecule selected from nucleic acids, preferably DNA or RNA, nucleotides, oligonucleotides, proteins and peptides, lipids, e.g. phospholipids, wherein preferably said biomolecule has a size > 0.1 kDa, e.g. 0.5kDa, preferably > 5kDa, more preferably >10kDa, even more preferably >50kDa, or wherein said analyte is a virus or a cel The sensor body according to any of the foregoing claims, wherein said interstitial pore space is dimensioned to accommodate liquid from a liquid sample containing or suspected of containing said analyte. The sensor body according to any of the foregoing claims, wherein said capture agent(s) is (are) specific for a given analyte and is(are) selected from antibodies, antibody fragments, nucleic acids, including aptamers, spiegelmers, non-antibody proteins capable of specifically binding an analyte or analyte complex, such as receptors, receptor fragments, affinity proteins, e.g. streptavidin, chemical moieties such as Biotin, a Strep-tag®, Digoxigenin, Dinitrophenol, a nucleic acid or nucleic acid analogue-tag or similar chemical moieties capable of being specifically bound, with an affinity in the range of from K D = 10 -8 to 10 -15 M, by antibodies, antibody fragments, nucleic acids, including aptamers, spiegelmers, non-antibody proteins, such as receptors, receptor fragments, affinity proteins, e.g. streptavidin, or is(are) selected from hydrophobic structures capable of specifically binding hydrophobic molecules or molecules with hydrophobic groups, wherein preferably, said hydrophobic structures have a logD greater than 2 under the conditions in which said detection of said analyte is performed. The sensor body according to any of the foregoing claims, which is either a solitary particle which preferably is freely diffusible, or said sensor body is a spot immobilized on a surface of a substrate. The sensor body according to claim 9, which is a solitary particle which preferably is freely diffusible, wherein said solitary particle has a spherical or globular shape, and preferably has a spherical or globular shape and clearly delimited boundaries that can be visibly observed, preferably in a microscope or other optical means. The sensor body according to any of claims 9 - 10, having an average diameter in the range of from 1µm to 1mm. The sensor body according to claim 9, which is a spot immobilized on a surface of a substrate, wherein said spot has clearly delimited boundaries that can be visibly observed, preferably in a microscope or other optical means, and preferably covers an average fixed area on the substrate in the range of from 1µm 2 to 1mm 2 , and wherein the thickness of said particle varies depending on the external conditions to which said spot is exposed and thus depending on whether said spot is in an expanded or a contracted state. A method of binding an analyte to a sensor body, said method comprising the steps: a) providing, in any order, a sensor body according to any of claims 1 - 12, and an aqueous sample suspected of containing an analyte; b) exposing said sensor body to said aqueous sample, thereby allowing an analyte present in said sample to bind to said sensor body and allowing liquid to enter said interstitial pore space of said sensor body. A method of enriching and/or washing an analyte bound to a sensor body, said method comprising the steps: c) Performing the method according to claim 13; d) Changing the state of said sensor body from an expanded to a contracted state and thereby displacing said liquid contained in said interstitial pore space of said sensor body, by changing at least one external condition to which said sensor body is exposed, e.g. one of temperature, pH, salt concentration, presence or absence of chemicals; e) Changing the state of said sensor body from a contracted to an expanded state and thereby allowing liquid to enter said interstitial pore space of said sensor body, by changing at least one external condition to which said particle is exposed, e.g. one of temperature, pH, salt concentration, presence or absence of chemicals; wherein steps d) and e) are performed after step c) when the analyte has been bound to said particle, and wherein the analyte is either labelled or unlabelled; wherein steps d) and e) are repeated n-times, wherein n is an integer in the range of from 1 - 1000, preferably 1 - 500, preferably 1 - 250, more preferably 1 - 100, even more preferably 1- 50 and even more preferably 1- 20. A method of detecting an analyte in a sample, said method comprising the steps: - Performing the method according to any of claims 13 - 14; wherein the analyte is or becomes labelled either before, during or after binding said analyte to said sensor body; - Detecting the analyte bound to said sensor body by detecting the label bound to said sensor body, preferably in the presence of unbound labe
CPC Classification	Investigating Or Analysing Materials By Determining Their Chemical Or Physical Properties Chemical Or Physical Processes; E.G. Catalysis Or Colloid Chemistry;Their Relevant Apparatus
Extended Family	049-304-421-486-610 039-023-624-397-083 009-275-671-001-662 141-375-034-472-633 027-168-502-614-360 193-715-825-466-066 157-462-969-488-055 172-806-193-126-806
Patent ID	3505934
Inventor/Author	Ellinger Thomas Ermantraut Eugen Steinmetzer Katrin Hubold Stephan Loncarevic Ivan Schulz Torsten
IPC	G01N33/543
Status	Discontinued
Simple Family	049-304-421-486-610 039-023-624-397-083 009-275-671-001-662 141-375-034-472-633 027-168-502-614-360 193-715-825-466-066 157-462-969-488-055 172-806-193-126-806
CPC (with Group)	G01N33/54306 G01N33/54313 B01J20/265
Issuing Authority	United States Patent and Trademark Office (USPTO)
Kind	Patent Application Publication