NDLI: A new microsieving technique in pollen analysis

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A new microsieving technique in pollen analysis

Content Provider	Birbal Sahni Institute of Paleobotany
Author	A., Stephen Anupama, K. Orukaimani, G. Prasad, S.
Abstract	A new procedure has been added to the standard pollen preparation protocols to address the issue of microdebris and clay removal to facilitate easier and better microscopic observation and counting. This procedure consists primarily of a technique that uses a 5μm membrane filter under water pressure to filter out particles < 5μm and concentrate polliniferous material from core sediments and surface samples. The procedure includes a brief (optional) ultrasound pre-treatment followed by filtration using a membrane microsieve, the innovation highlighted herein. This technique is observed to be efficient in palynomorph retrieval from different kinds of sediment samples - the quantitative efficiency being superior to that of the conventional procedure and comparing favourably with ultrasonic microfiltration. The paper describes the new method, discusses its advantages and provides an illustrative, quantitative and qualitative comparison with the usual method (without microsieving) and recommends its widespread adoption in quantitative palynological studies.
Starting Page	551
Ending Page	557
File Format	PDF
Volume Number	57
Language	English
Publisher Date	2008-01-01
Access Restriction	Open
Subject Keyword	Pollen analysis Microdebris Microsieving Membrane filter
Content Type	Text
Educational Use	Research Reading
Resource Type	Article
Education Level	Under Graduate Post Graduate
Subject	Paleobotany; fossil microorganisms

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